A Guest Of Honour by Nadine Gordimer

By Nadine Gordimer

James Bray, an English colonial administrator who was once expelled from a valuable African kingdom for siding with its black nationalist leaders, is invited again ten years later to affix within the country's independence celebrations. As he witnesses the factionalism and violence that erupt as innovative beliefs are subverted through ambition and greed, Bray is once more compelled to settle on aspects, a decision that turns into either his triumph and his undoing.

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2001) Tie-1-directed expression of Cre recombinase in endothelial cells of embryoid bodies and transgenic mice. J. Cell Sci. 114, 671–676. 47. 47 Emambokus, N. R. and Frampton, J. (2003) The glycoprotein IIb molecule is expressed on early murine hematopoietic progenitors and regulates their numbers in sites of hematopoiesis. Immunity 19, 33–45. 48. 48 Mikkola, H. , Schlaeger, T. , et al. (2003) Haematopoietic stem cells retain long-term repopulating activity and multipotency in the absence of stem-cell leukaemia SCL/tal-1 gene.

Et al. (1992) Targeted oncogene activation by site-specific recombination in transgenic mice. Proc. Natl. Acad. Sci. USA 89, 6232–6236. 67. , Yeoh, E. , and Downing, J. R. (2002) Expression of a conditional AML1-ETO oncogene bypasses embryonic lethality and establishes a murine model of human t(8;21) acute myeloid leukemia. Cancer Cell 1, 63–74. 68. 68 Lam, K. , and Rajewsky, K. (1997) In vivo ablation of surface immunoglobulin on mature B cells by inducible gene targeting results in rapid cell death.

Reporter” or indicator alleles offer an alternative approach to monitoring Cre-mediated excision, particularly at the single cell level. , the ROSA26 locus (72–74). Expression of the marker gene such as lacZ, GFP, and enhanced yellow fluorescent protein is activated upon Cre-mediated removal of the stopper fragment and can be monitored by immunohistochemistry or flow cytometry (44,65,74–76). The marker locus is introduced into a conditional knockout background by breeding the conditional, Cre-expressing, and reporter strains.

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