By A H Rebar; et al
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Extra resources for A Guide to Hematology in Dogs and Cats
Cells touching bottom and right center lines are not counted. Double ruling: Cells touching top and left outer lines are counted. Cells touching bottom and right inner lines are not counted. , Ames, IA, the Iowa State University Press, 1978 Figure 3-11 Unopette® diluting pipette and reservoir for counting WBCs and platelets in a hemocytometer. 20 TNM GuideToHema:03058 TNM text_cp 6/28/07 3:49 PM Page 21 3 LABORATORY METHODS IN HEMATOLOGY ® Platelet count – Can be performed using hemocytometer and ammonium oxalate diluent but it is difficult to achieve accuracy even among skilled medical technologists.
If autoagglutination is present, the diagnosis of immune hemolysis is confirmed without running the Coombs’ test. 46 TNM GuideToHema:03058 TNM text_cp 6/28/07 3:49 PM Page 47 4 E RY T H R O C Y T E S Figure 4-25 Canine blood. Spherocytosis. The smaller RBCs that lack central pallor are spherocytes. These cells are frequent in dogs with immune-mediated hemolytic disease. They can also occur in fragmentation hemolysis. A ghost RBC (arrow) is noted in the center of the field and suggests some degree of intravascular lysis (100x).
39 TNM GuideToHema:03058 TNM text_cp 6/28/07 3:49 PM Page 40 Artifacts Crenation: the presence of red cells covered by short spiky surface projections (Figure 4-18). ® Crenation is the most common artifactual change seen in blood films. ® Crenation can be confused with acanthocytic change. ® Crenation is more prominent in films made from EDTA blood. ® Crenation is differentiated from true poikilocytosis in that crenation affects all the red cells in a given area of the film whereas true poikilocytosis affects only scattered red cells on the blood film.